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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dirofilaria immitis Pepsin inhibitor Dit33 (DIT33)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dirofilaria immitis Pepsin inhibitor Dit33 (DIT33)品名简称:
Recombinant Dirofilaria immitis DIT33 protein货号:
CSB-EP638886DKS规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
DIT33Uniprot No.:
Q27384别名:
/种属:
Dirofilaria immitis (Canine heartworm)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
32.0 kDa表达区域:
18-234aa氨基酸序列:
SVINRHNKRFAGFSVAGIGGTAGCVVVDNKLFANSFYLRDLTTEEQRELAQYVEDSNQYKEEVKTSLEERRKGWQLARHGEKDAKVLSSLAEKKFPKPPKKPSFCSAGDTTQYYFDGCMVQNNKIYVGRMYVRDLTSDEINQLKTFDAKMTAYQKYLSSSIQQQVDSLFGDKSNLFNLFTDTRHETSSQPSDATTISTTTQAPVEPPETPHFCIAIY蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Aspartyl protease inhibitor.功能2:
/文献:
"Cloning and expression of DiT33 from Dirofilaria immitis: a specific and early marker of heartworm infection." Hong X.Q., Mejia J.S., Kumar S., Perler F.B., Carlow C.K.S. Parasitology 112:331-338(1996)研究领域:
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文献和实验层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源
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