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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Isoleucine--tRNA ligase, cytoplasmic (IARS), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3468.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
IARSUniprot No.:
P41252别名:
Isoleucyl-tRNA synthetase;IRS;IleRS种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
26.3 kDa表达区域:
693-852aa氨基酸序列:
DRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVV蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验第一节 工具酶 基因工程的基本技术是人工进行基因的剪切、拼接、组合。基因是一段具有一定功能的DNA分子,要把不同基因的DNA线形分子片段准确地切出来,需要各种限制性核酸内切酶(restriction endonuclease);要把不同片段连接起来,需要DNA连接酶(ligase);要结合基因或其中的一个片段,需要DNA酶(DNa polymerase)等。因此,酶是DNA重组技术中必不可少的工具,基因工程中所要用的酶统称为工具酶。 一、DNA限制
DNA重组技术及其在基因诊断中的应用 工具酶 基因工程的基本技术是人工进行基因的剪切、拼接、组合。基因是一段具有一定功能的DNA分子,要把不同基因的DNA线形分子片段准确地切出来,需要各种限制性核酸内切酶(restriction endonuclease);要把不同片段连接起来,需要DNA连接酶(ligase);要结合基因或其中的一个片段,需要DNA酶(DNa polymerase)等。因此,酶是DNA重组技术中必不可少的工具,基因工程中所要用的酶
type I escape from RNA interference, j. Virol., 77, 11531, 2003. 20. Srivastava, A., Obstacles to human hematopoietic stem cell transduction by recombinant adeno-associated virus 2 vectors, J. CellBiochem. Suppl., 38, 39, 2002. 21. McTaggart, S
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