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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase (P2), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase (P2), partial品名简称:
Recombinant Pseudomonas phage phi6 RNA-directed RNA polymerase protein, partial货号:
CSB-EP319967PUV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
P2Uniprot No.:
P11124别名:
Protein P2种属:
Pseudomonas phage phi6 (Bacteriophage phi-6)蛋白长度:
Partial来源:
E.coli分子量:
27.2 kDa表达区域:
310-485aa氨基酸序列:
NKEEKVKEWSLCVATDVSDHDTFWPGWLRDLICDELLNMGYAPWWVKLFETSLKLPVYVGAPAPEQGHTLLGDPSNPDLEVGLSSGQGATDLMGTLLMSITYLVMQLDHTAPHLNSRIKDMPSACRFLDSYWQGHEEIRQISKSDDAILGWTKGRALVGGHRLFEMLKEGKVNPSP蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Rna-dependent RNA polymerase part of the packaging complex that packages the viral RNA segments, replicate them into a double-stranded form and transcribe them.功能2:
/文献:
"Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase." Mindich L., Nemhauser I., Gottlieb P., Romantschuk M., Carton J., Frucht S., Strassman J., Bamford D.H., Kalkkinen N. J. Virol. 62:1180-1185(1988)研究领域:
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文献和实验-HCl, 1mM EDTA, pH8.5,RNase-free。RNA 溶解溶液,室温密闭贮存。Buffer G-A:裂解液,溶解蛋白质-基因组DNA 复合物,释放基因组DNA。室温密闭贮存。Buffer G-B:蛋白质变性剂,室温密闭贮存。Buffer P2:分相溶液,室温密闭贮存。Buffer B:DNA 结合溶液,室温密闭贮存。Buffer W1:洗涤液,室温密闭贮存。Buffer W2:脱盐液,使用前按试剂瓶上指定体积加入无水乙醇,混合均匀,室温密闭贮存。Eluent:2.5mM Tris
影响。随着洗脱体积的增大回收率增高,但产品浓度降低。为了得到较高的回收率可以增大洗脱体积。 12. 洗脱时间过短:洗脱时间对回收率也会有一定影响。洗脱时放置1分钟可达到较好的效果。 Q: 质粒纯度不高,如何解决? A: 1. 混有蛋白:不要使用过多菌体。经溶液P1、P2、P3处理,离心后溶液应为澄清的,如果还混有微小蛋白悬浮物可再次离心去除后再进行下一步骤。 2. 混有RNA:RNaseA处理不彻底,请减少菌体用量或加入溶液P3之后室温放置一段时间。如果溶液P1已保存6个月
(to amplify cDNA by the polymerase chain reaction). The RT reaction involves synthesizing cDNA from total RNA or polyA enriched RNA (mRNA). This cDNA is now ready to amplify by PCR. Using specific oligonucleotides (i.e. primers) a fragment of the cDNA
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