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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Salmonella enteritidis Cold shock protein CspA (cspA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Salmonella enteritidis Cold shock protein CspA (cspA)品名简称:
Recombinant Salmonella enteritidis cspA protein货号:
CSB-EP359368SBG规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
cspAUniprot No.:
P0A9Y5别名:
7.4 kDa cold shock protein CS7.4种属:
Salmonella enteritidis蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.7 kDa表达区域:
2-70aa氨基酸序列:
SGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKSLDEGQKVSFTIESGAKGPAAGNVTSL蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3' (By similarity).功能2:
/文献:
/研究领域:
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文献和实验Cold-Inducible Promoters for Heterologous Protein Expression
, often synergistic, adaptive strategies to handle stress. In the case of cold shock, the need for restoring transcription and translation is handled by an immediate increase in the synthesis of about 16 cold shock proteins (Csps) (4 ), while the cell solves
Strategies to Optimize Protein Expression in E. coli
experimental analysis. J. Mol. Biol. 336:115‐130. Goldstein, J., Pollitt, N.S., and Inouye, M. 1990. Major cold shock protein of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87
Expression and Purification of Hepatitis C Virus Protease from Clinical Samples
3 protein from patients under therapy of protease inhibitors. It gives the details of expression and purification of NS3 protease using the pCold vectors that contains a promoter derived from the cold-shock genes to drive the expression of NS3 protein
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