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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Salmonella enteritidis Cold shock protein CspA (cspA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
cspAUniprot No.:
P0A9Y5别名:
7.4 kDa cold shock protein CS7.4种属:
Salmonella enteritidis蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.7 kDa表达区域:
2-70aa氨基酸序列:
SGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKSLDEGQKVSFTIESGAKGPAAGNVTSL蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Binds to and stimulates the transcription of the CCAAT-containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3' (By similarity).内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
TranscriptionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验【求助】Cysteine string protein (CSPa)是什么蛋白
cindy10moon 写综述,看到一篇文献提到Cysteine string protein (CSPa),说是HSP40家族中的一员,哪位高人知道怎么翻译?说和神经再生和突触传导相关,小妹***! zhujoker 半胱氨酸串联蛋白质好像比较合适了,自己再找找吧 cindy10moon zhujoker wrote: 半胱氨酸串联蛋白质好像比较合适
Strategies to Optimize Protein Expression in E. coli
experimental analysis. J. Mol. Biol. 336:115‐130. Goldstein, J., Pollitt, N.S., and Inouye, M. 1990. Major cold shock protein of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87
整合宿主因子,一种与DNA结合的序列特异性多功能蛋白来正调控[45,46]。 主要冷应激基因cspA启动子同样也被证明在低温具有活性[47]。对cspA和PL启动子进行分子剖析,鉴定了在较低温度下参与增强转录的特异性DNA区域。从而开发了一系列在20℃低温具有高活性的PL衍生启动子[48]。选用冷反应启动子的基本原理是在15-20℃的条件下,蛋白质的折叠速度只受到轻微的影响,而作为生物化学反应,转录和翻译的速度将被充分降低。 这就为蛋白质折叠、产生有活性的蛋白和避免非活性蛋白
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