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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Vaccinia virus Protein A27 (VACWR150)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
VACWR150Uniprot No.:
P11258别名:
/种属:
Vaccinia virus(strain Western Reserve)(VACV)(Vaccinia virus(strain WR))蛋白长度:
Full Length来源:
E.coli分子量:
17.6 kDa表达区域:
1-110aa氨基酸序列:
MDGTLFPGDDDLAIPATEFFSTKAAKKPEAKREAIVKADEDDNEETLKQRLTNLEKKITNVTTKFEQIEKCCKRNDEVLFRLENHAETLRAAMISLAKKIDVQTGRRPYE蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
othersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"The vaccinia virus A27L protein is needed for the microtubule-dependent transport of intracellular mature virus particles." Sanderson C.M., Hollinshead M., Smith G.L. J. Gen. Virol. 81:47-58(2000)Function:
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文献和实验L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白,兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G,也可根据结合力表格进行选择,详见资源信息部分 3、基质—琼脂糖还是磁珠? 琼脂糖 磁珠 优点 简单易用,无需辅助设备 操作简单 孔径大,直径大(50-150
Construction and Characterization of Adenovirus Vectors
. Infect a 150-mm dish of 293 cells with 1 mL of inoculum. Return cells to the incubator. xii. Allow the virus to adsorb for 1 h, rocking the dishes every 10-15 min. xiii. After the adsorption period
Protocol of the protein extraction method
of the protein. Annotation: the component of buffer: Lysis bufferⅠ (250ml): Glycerd: 25ml (10%) Triton X-100: 2.5ml (1%) Hepes (PH7.5): 12.5ml (50mM) NaCl (150mM): 37.5ml NaF (2mM) EDTA
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