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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 1 (hmw1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mycoplasma pneumoniae Cytadherence high molecular weight protein 1 (hmw1), partial品名简称:
Recombinant Mycoplasma pneumoniae hmw1 protein, partial货号:
CSB-EP700522MLW规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
hmw1Uniprot No.:
Q50365别名:
Cytadherence accessory protein 1种属:
Mycoplasma pneumoniae (strain ATCC 29342 / M129)蛋白长度:
Partial来源:
E.coli分子量:
22.0 kDa表达区域:
1-139aa氨基酸序列:
MKKSKEAVFEDKDYTEENPEQIFGNLYDGKLTVQDGKVKIAYDGDGNGYYIAFNSETGVYYDPYGDTEYDISVLFDANGNSFVFADAPTVEVLAGEQEQTEAEPDYLQYVGNEAYGYYDEAGEWVWSGY蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Component of the cytoskeleton-like structure which stabilizes the shape of the wall-less Mycoplasma. This cytoskeleton-like network of accessory proteins containing HMW proteins 1 to 5 allows the proper anchoring of cytadhesin proteins in the mycoplasmal membrane at the attachment organelle (By similarity).功能2:
/文献:
"Juxtaposition of the genes encoding Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW3." Krause D.C., Lee K.K. Gene 107:83-89(1991)研究领域:
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文献和实验Subculture of Suspension Cell Lines
are obtained within 4 weeks. Mycoplasma colonies observed on agar plates have a `fried egg? appearance (see Mycoplasma pneumoniae',' ');">Figure 14). Materials 70% ethanol in water (Prod. No. R8382) Mycoplasma Pig Agar plates (in 5cm petri dishes
Subculture of Suspension Cell Lines
agar plates after 14 days incubation at x300 magnification using an inverted microscope for the presence of mycoplasma colonies (see Figure 14 ). Criteria for a Valid Result All positive control agar plates and broths show evidence
incubation, subculture 0.1 ml of test broth onto an agar plate and incubate plate anaerobically as above. Observe agar plates after 14 days incubation at x300 magnification using an inverted microscope for the presence of mycoplasma colonies (see Figure 14
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