aprN/aprN蛋白Recombinant Bacillu

纯度：

Greater than 85% as determined by SDS-PAGE.

基因名：

aprN

Uniprot No.：

P35835

别名：

Nattokinase

种属：

Bacillus subtilis subsp. natto

蛋白长度：

Full Length of Mature Protein

来源：

E.coli

分子量：

31.8 kDa

表达区域：

107-381aa

氨基酸序列：

AQSVPYGISQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDG_S_SHGTHVAGTIAALNNSIGVLGVAPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPTGSTALKTVVDKAVSSGIVVAAAAGNEG_S_SGSTSTVGYPAKYPSTIAVGAVNSSNQRASFSSVGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ

蛋白标签：

N-terminal 6xHis-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

13-23 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity. Miscellaneous Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. In Japan this enzyme, isolated from a process of fermentation involving boiled soybeans and Bacillus natto, is used as a food supplement because it is reported to reduce and prevent blood clotting. Catalytic activityi Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. EC:3.4.21.62

内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

Developed Protein

Biological_Activity:

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Research Areas:

Epigenetics and Nuclear Signaling

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto." Yanagisawa Y., Chatake T., Chiba-Kamoshida K., Naito S., Ohsugi T., Sumi H., Yasuda I., Morimoto Y. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66:1670-1673(2010)

Function:

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