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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Leukemia inhibitory factor (LIF) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Leukemia inhibitory factor (LIF) (Active)品名简称:
Recombinant Human LIF protein (Active)货号:
CSB-MP012928HUd9规格:
20μg价格:
828LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
Measured by its binding ability in a functional ELISA. Immobilized human LIF at 2 μg/ml can bind human LIFR (CSB-MP012929HUi9), the EC50 is 22.58-30.24 ng/ml.基因名:
LIFUniprot No.:
P15018别名:
/种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
48.6 kDa表达区域:
23-202aa氨基酸序列:
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF蛋白标签:
C-terminal hFc1-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
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文献和实验, however, by the inhibitory protein I-KB. In B lymphocytes, NF-KB is released from I-KB and moves to the nucleus, where it activates transcription. • A dimeric factor may have alternative partners. One partner may cause it to be inactive; synthesis of the active
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
medium for OP9 cells (for Protocols 1, 2) Leukemia inhibitory factor (LIF; 10 µg/mL; Chemicon LIF2010) (for Protocol 2; see Step 20) Lympholyte-M (Cedarlane CL5120) (for Protocol 3) Mice, 4-8 wk old (for Protocol 4; see Step 69) Mice, fetal
Maintenance of Chicken Embryonic Stem Cells In Vitro
In this chapter, we describe the methods we have used to show that chicken leukemia inhibitory factor (LIF) maintains chicken embryonic stem (ES) cells in an undifferentiated state in culture. Recombinant chicken LIF (rchLIF) was expressed
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