TNFSF13B/TNFSF13B蛋白Recombinant

纯度：

Greater than 90% as determined by SDS-PAGE.

基因名：

TNFSF13B

Uniprot No.：

Q9Y275

别名：

B lymphocyte stimulator （BLyS） （B-cell-activating factor） （BAFF） （Dendritic cell-derived TNF-like molecule） （TNF- and APOL-related leukocyte expressed ligand 1） （TALL-1）

种属：

Homo sapiens (Human)

蛋白长度：

Partial

来源：

Mammalian cell

分子量：

46.6 kDa

表达区域：

134-285aa

氨基酸序列：

AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

蛋白标签：

N-terminal hFc-tagged

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.

内毒素：

Less than 1.0 EU/ug as determined by LAL method.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

Active Protein & In Stock Protein

Biological_Activity:

①Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 μg/ml can bind human BCMA (CSB-MP023974HU1), the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.②Human SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind Human CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay.③Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 μg/ml can bind TNFRSF13C（CSB-MP853495HU1）, the EC50 is 9.943-15.72 ng/ml.

Research Areas:

Cancer

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

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Function:

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