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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Somatotropin (GH1) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥22200.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2568.0 |
| 规格: | 20μg | 产品价格: | ¥1368.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
GH1Uniprot No.:
P01241别名:
Growth hormone (GH) (GH-N) (Growth hormone 1) (Pituitary growth hormone)种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Mammalian cell分子量:
27.2 kDa表达区域:
27-217aa氨基酸序列:
FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Growth hormone (GH) (GH-N) (Growth hormone 1) (Pituitary growth hormone)内毒素:
Less than 1.0 EU/ug as determined by LAL method.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
①Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.Research Areas:
Developmental BiologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验二硫键的形成)和NaCl[5]。 3、 供给丰富的培养基,创造最佳培养条件,如供氧、pH等。 包涵体的分离及溶解 对于生物制药工业来说,包涵体的形成也是有利的,不仅可获得高表达、高纯度的重组蛋白质,还可避免细胞水解酶对重组蛋白质的破坏。由于包涵体是蛋白质聚集而成的致密颗粒,分离的第一步是对培养收集的细胞进行破碎,比较有效的方法是高压匀浆结合溶菌酶处理,然后5000~20000g离心,可使大部分包涵体沉淀,与可溶性蛋白分离,接着,包涵体沉淀需用去污剂(Triton
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
.,active[143Yellowjacket and paper wasp venom allergen A5 S, 100 mg/L, α-MF360Vertebrates (non-human)Bovine β-caseinI, 1 g/L[150]Bovine enterokinase catalytic domainS, 6.3 mg/L, α-MF[144]Bovine follicle stimulating hormone β-subunitS, 4 mg/ml, α-MF[145
Diabetes mellitus Gonadotropin deficiency Growth hormone deficiency
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