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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Carbonic anhydrase 1 (CA1) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/500μg/50μg
| 规格: | 1mg | 产品价格: | ¥25524.0 |
|---|---|---|---|
| 规格: | 500μg | 产品价格: | ¥17868.0 |
| 规格: | 50μg | 产品价格: | ¥2088.0 |
纯度:
Greater than 95% as determined by SDS-PAGE.基因名:
CA1Uniprot No.:
P00915别名:
Carbonic Anhydrase 1; Carbonate Dehydratase I; Carbonic Anhydrase B; CAB; Carbonic Anhydrase I; CA-I; CA1种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
29.93 kDa表达区域:
2-261aa氨基酸序列:
ASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid缓冲液:
0.2 μm filtered 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Carbonic Anhydrase 1 (CA1) is a cytosolic enzyme, belonging to the alpha-carbonic anhydrase family. It is highly expressed in erythrocytes and acts as an early marker for erythroid differentiation. Carbonic anhydrase 1 plays a improtant role in many biological processes such as calcification, cellular respiration, bone resorption, acid-base balance. It is activated by imidazole, histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. At the same time, It is inhibited by sulfonamide derivatives and coumarins. In addition, CA1 is a zinc metalloenzyme that has reversible hydration of carbon dioxide. It can hydrate cyanamide to urea.内毒素:
Less than 1.0 EU/µg as determined by LAL method.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
The esterase activity is determined to be greater than 500 pmol/min/ugResearch Areas:
Cell BiologyReconstitution:
/Reference:
/Function:
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
spectroscopy–based proteomics, we detected 117 proteins in human vitreous and elevated levels of extracellular carbonic anhydrase-I (CA-I) in vitreous from individuals with diabetic retinopathy, suggesting that retinal hemorrhage and erythrocyte lysis
中科院高福院士课题组 2020 年发表了哪些重要的研究成果?
隆抗体 CB6 正式产品名为 JS016,目前已进入临床三期试验,前期数据表明能够有效治疗新冠轻中症感染患者。 图源:中科院微生物研究所 10、设计通用策略研发新冠病毒重组蛋白疫苗DOI: 10.1016/j.cell.2020.06.035疫苗可以说是解决新冠病毒蔓延最好的方式,新冠肺炎疫请发生后各国科学家都在争分夺秒地研究新冠疫苗,中国的疫苗研发也是一直处于世界前列。2020 年 8 月 6 日,高福院士联合严景华等在 Cell 杂志发文:A Universal Design
The Application of the Chemical Array for Biological Study
library GLORIA (Gene Library of Osada Laboratory at RIKEN for chemical array analysis) has been established. Using the systematic platform, we detected novel inhibitors of carbonic anhydrase II. We also have shown that this screening method is useful
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