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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-13 (IL13), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10μg
英文名称:
Recombinant Human Interleukin-13 (IL13), partial (Active)品名简称:
Recombinant Human IL13 protein, partial (Active)货号:
CSB-AP004321HU规格:
10μg价格:
1740LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Less than 1.0 EU/μg as determined by LAL method.生物活性:
The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 1.5-4.5 ng/ml.基因名:
IL13Uniprot No.:
AAH96139别名:
Interleukin-13;IL-13;种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Mammalian cell分子量:
13.4 kDa表达区域:
35-146aa氨基酸序列:
GPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGQFN蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.功能2:
Cytokine文献:
/研究领域:
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文献和实验【讨论】Innate immunity: 'Natural helper' cells identified
lineage (LIN) markers but express interleukin-7 receptor-α (IL-7Rα) and ST2 (a subunit of IL-33R), along with several other cell surface molecules. Further analysis showed that these cells are of lymphoid lineage but are distinct from B and T cells
Immunity:「填补教科书」董晨院士团队解析 IL-17D 重要功能,并首次发现其受体
慢性炎症与自身免疫性疾病、过敏性疾病和肿瘤的发展密切相关,炎性因子白介素 - 17(IL-17)家族在促进宿主防御和自身免疫性疾病中起着重要作用。IL-17 家族由六个成员组成(包括 IL-17A、B、C、D、E、F),IL-17D 是 IL-17 家族中了解最少的成员,其受体也尚不明确。 图片来源:Frontiers in Immunology(1)2021 年 4 月 13 日,清华大学医学院董晨院士团队在 Immunity 上在线发表了题为 Interleukin-17
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
[4] 。由于在信号转导和表观遗传研究中的突出作用,ChIP 在肿瘤 [5-7] 、神经科学 [8-10] 、植物发育 [11-13] 等领域中应用非常广泛,同时有关细胞凋亡 [14] 、雌激素信号转导 [15] 、胰岛素抵抗 [16] 、组织发育 [1] 的文献中也用到ChIP。 目前,最常见的有以下两种 ChIP 实验技术: nChIP:用来研究 DNA 及高结合力蛋白,采用微球菌核酸酶(micrococcal nuclease)消化染色质,然后进行片段富集及后续分析,适用于组蛋白及其异构体,例如 [17
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