EGF/EGF蛋白Recombinant Human Pro

纯度：

Greater than 95% as determined by SDS-PAGE.

基因名：

EGF

Uniprot No.：

P01133

别名：

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

种属：

Homo sapiens (Human)

蛋白长度：

Partial

来源：

E.coli

分子量：

6.2 kDa

表达区域：

971-1023aa

氨基酸序列：

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

蛋白标签：

Tag-Free

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 200 mM NaCl, pH 8.0

货期：

5-10 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

内毒素：

Less than 1.0 EU/µg as determined by LAL method.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

/

Product types:

Active Protein & In Stock Protein

Biological_Activity:

The ED50 as determined in a cell proliferation assay using BALB/c 3T3 cells is 60-450 pg/ml.

Research Areas:

Signal Transduction

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

/

Function:

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro