N/N蛋白/Nucleocapsid protein;NC;

Research Areas

Microbiology

Uniprot ID

Q8V432

Gene Names

N

Alternative Name(s)

Nucleocapsid protein;NC;Protein N

Lead Time

3-7 business days

Organism

Bovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)

Source

E.coli

Expression Region

1-448aa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Target Protein Sequence

MSFTPGKQSSSRASSGNRSGNGILKWADQSDQSRNVQTRGRRAQSKQTATSQQPSGGNVVPYYSWFSGITQFQKGKEFEFAEGQGVPIAPGVPATEAKGYWYRHNRRSFKTADGNQRQLLPRWYFYYLGTGPHAKDQYGTDIDGVYWVASNQADVNTPADILDRDPSSDEAIPTRFPPGTVLPQGYYIEGSGRSAPNSRSTSRASSRASSAGSRSRANSGNRTPTSGVTPDMADQIASLVLAKLGKDAAKPQQVTKQTAKEIRQKILNKPRQKRSPNKQCTVQQCFGKRGPNQNFGGGEMLKLGTSDPQFPILAELAPTAGAFFFGSRLELAKVQNLSGNLDEPQKDVYELRYNGAIRFDSTLSGFETIMKVLNENLNAYQQQDGTMNMSPKPQRQRGQKNGQGENDNISVAAPKSRVQQNKIRELTAEDISLLKKMDEPFTEDTSEI

MW

50.2 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Form

Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid form

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.