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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在零下15度以下保存, 避免光照
- 保质期:
2年
- 英文名:
Helixyte™ 14 [Equivalent to SYBR 14] *5 mM*
- 库存:
现货
- 供应商:
AAT Bioquest
- 规格:
0.5 mL
| Ex (nm) | 489 | Em (nm) | 520 |
| 分子量 | 474.06 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称:Helixyte 14
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:462.35
外观:固体
溶剂:DMSO
激发波长(nm):489
发射波长(nm):520
产品介绍
Helixyte 14具有与SYBR 14相同的化学结构(SYBR是ThermoFisher的商标)。 它通常用于检测精子的活性。 精液中活精子的比例可通过使用SYBR-14和碘化丙啶(PI)的双重染色技术进行评估。 Helixyte 14是一种核酸染料,与DNA结合后可在488 nm处大吸收并在518 nm处发射。 它使活精子的细胞核染成绿色。 相反,PI仅染色失去其膜完整性的非活动性精子。 可以通过先用SYBR-14和PI染色,然后通过流式细胞仪评估染色吸收来确定活精和死精的比例。 通过使用SYBR-14和PI双重染色,可以很容易地确定哺乳动物精液中活的和死的精子的比例,并可以通过流式细胞术进行定量。
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文献和实验Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender.
Authors: Hermansson, Ulrika and Johannisson, Anders and Axnér, Eva
Journal: Veterinary medicine and science (2021)
Evaluation of the use of SYBR-14 and propidium iodide stain in a fluorescent computer-assisted spermatozoal quantification method in dogs.
Authors: Watts, John R
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 89-102
Measurement of membrane integrity in canine spermatozoa using a fluorescent computer-assisted spermatozoal quantification method after SYBR-14/PI staining compared with manual counting after CFDA/PI staining.
Authors: Watts, John
Journal: Reproduction in domestic animals = Zuchthygiene (2021)
The impact of five years storage/biobanking at -80°C on mouse spermatozoa fertility, physiology, and function.
Authors: Raspa, Marcello and Putti, Sabrina and Paoletti, Renata and Barboni, Barbara and Ramal-Sanchez, Marina and Lanuti, Paola and Marchisio, Marco and D'Atri, Mario and Ortolani, Claudio and Papa, Stefano and Valbonetti, Luca and Bernabo, Nicola and Scavizzi, Ferdinando
Journal: Andrology (2021)
Addition of butylated hydroxytoluene (BHT) in tris-based extender improves post-thaw quality and motion dynamics of dog spermatozoa.
Authors: Sun, Lingwei and Wu, Caifeng and Xu, Jiehuan and Zhang, Shushan and Dai, Jianjun and Zhang, Defu
Journal: Cryobiology (2020): 71-75
Butaphosphan and Cyanocobalamin Supplementation in Semen Extender on Chilled Boar Sperm Quality and Life Span.
Authors: Suwimonteerabutr, J and Chumsri, S and Tummaruk, P and Nuntapaitoon, Morakot
Journal: Frontiers in veterinary science (2020): 592162
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity.
Authors: Bernal, B and Iglesias-Cabeza, N and Sánchez-Rivera, U and Toledano-Díaz, A and Castaño, C and Pérez-Cerezales, S and Gutiérrez-Adán, A and López-Sebastián, A and García-Casado, P and Gil, M G and Woelders, H and Blesbois, E and Santiago-Moreno, J
Journal: Poultry science (2020): 7133-7141
Resveratrol protects boar sperm in vitro via its antioxidant capacity.
Authors: Sun, Linlin and Fan, Xiaoteng and Zeng, Yao and Wang, Liqian and Zhu, Zhendong and Li, Rongnan and Tian, Xiue and Wang, Yongjun and Lin, Yan and Wu, De and Zeng, Wenxian
Journal: Zygote (Cambridge, England) (2020): 1-8
Soy lecithin as a potential alternative to powdered egg yolk for buck sperm cryopreservation does not protect them from mitochondrial damage.
Authors: Tabarez, Abigail and García, Wilber and Palomo, María Jesús
Journal: Animal reproduction science (2020): 106473
The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa.
Authors: Schäfer-Somi, Sabine and Budik, Sven
Journal: Acta veterinaria Scandinavica (2020): 2
时发现,这个实验其实是有扩增的,但原本应该平直的 ROX 荧光曲线却呈现出一个跟 SYBR 颠倒对称的图形(图 12B)。我们查看 Raw data 时发现其第一通道荧光(FAM)是正常上升的,第四通道荧光(ROX)也是维持在正常水平,这就怀疑可能与校准文件异常有关。这台仪器在重新做了 SYBR 荧光校准以后,扩增曲线就恢复正常了,也能得到正常的 CT 值。 图 12 校准文件异常导致曲线异常 关于扩增曲线异常的更多案例分析可以参考我们之前的推文《荧光定量 PCR 异常扩增曲线分析攻略
实验步骤 标记目标红细胞: 根据选择的DNA染料选择对目标红细胞的荧光标记,这本身就取决于流式细胞仪在入侵检测分析结束时获得的数据,CFDA SE的荧光发射峰约为517nm,接近520nm处SYBR Green I的荧光。CFDASE可以与Hoechst 33342组合使用,DDAO SE 的荧光发射峰接近657nm。因此,它可以与Hoechst 33342或SYBR Green I组合使用。 1.加入12mL
Thermostable DNA polymerases 37 3.11 Properties of Taq DNA polymerase 37 3.12 Thermostable proofreading DNA polymerases 43 3.13 Tth DNA polymerase has reverse transcriptase activity 46 3.14 Red and green polymerases and reagents 47 3.15 Polymerase mixtures
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