相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
根据瓶身LOT号查询
- 英文名:
Zymosan A from Saccharomyces cerevisiae
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
58856-93-2
- 规格:
250MG
属性
生物来源
Saccharomyces cerevisiae
质量水平
200
表单
powder with small lumps
技术
flow cytometry: suitable
tissue culture: suitable
颜色
off-white to light brown
储存温度
2-8°C
应用
- 用作阳性对照品,确定激活补体级联反应
- 检测呼吸爆发(RB)
- 评估热应激(HS)下,其对奶牛产奶性能、免疫力、热休克蛋白和凋亡相关基因表达的影响
- 用作克雷布斯-林格(Krebs-Ringer)磷酸盐(KRP)培养基中的刺激剂
- 诱导小鼠发生热痛觉过敏
- 用流式细胞仪
- 研究其对虹鳟鱼(Oncorhynchus mykiss)头肾白细胞的影响
生化/生理作用
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2.
C-type lectin receptors (CLRs) elicit immune responses upon recognition of glycoconjugates present on pathogens and self-components. While Dectin-1 is the best-characterized CLR recognizing β-glucan on pathogens, the endogenous targets of Dectin-1 are not fully understood. Herein, we report that human Dectin-1 is a ligand for CLEC-2, another CLR expressed on platelets. Biochemical analyses revealed that Dectin-1 is a mucin-like protein as its stalk region is highly O-glycosylated. A sialylated core 1 glycan attached to the EDxxT motif of human Dectin-1, which is absent in mouse Dectin-1, provides a ligand moiety for CLEC-2. Strikingly, the expression of human Dectin-1 in mice rescued the lethality and lymphatic defect resulting from a deficiency of Podoplanin, a known CLEC-2 ligand. This finding is the first example of an innate immune receptor also functioning as a physiological ligand to regulate ontogeny upon glycosylation.
)(库存货号:Fluka A2427-250G) 40g/L 葡萄糖dextrose(国药 14434-43-7) 14g/L YNB Yeast Nitrogen Base with ammonium sulfate w/out amino acids (SIGMA Y0626) 5.4 g/L Na2HPO4(国药10039-32-4) 7.4 g/L NaH2PO4(国药14431-43-7) 44 2)用于YVH10
) 酸洗玻璃珠,美国Sigma公司。 11) 其他试剂为国产分析纯。 2. 培养基与常用溶液 1) YPD培养基 酵母提取物 10 g 蛋白胨 20 g 溶于900 mL水中,高压灭菌20 min,冷却后,加入100 mL无菌的20%葡萄糖储存液。室温下可保存两个月。 2) YPDA培养基 酵母提取物
) 。 ( 2 ) 提取介质:被收集的植物材料置于提取介质中。提取介质是为稳定亚细胞馏分的混合试剂。依照 Greimer 和 Deltoid [13」所述并稍作改动:2% 阿拉伯树胶(Sigma) 、1.25% Ficoll (Sigma) 、2.5% 硫酸葡萄聚糖(Fluka) 、25 mmol/L Tris-HCl ( pH 7.4)、0.5 mmol/L EDTA、2.5 mmol/L MgCl2、4 mmol/L 正辛醇、8 mmol/L β- 疏基乙醇 ( Sigma) 、6.8 mmol/L 二乙基焦
技术资料暂无技术资料 索取技术资料









