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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
γ免疫球蛋白 Fc 段
- 形态:
液体
- 保存条件:
4℃长期保存
- 适应物种:
人
- 保质期:
4℃长期保存
- 库存:
现货充足
- 供应商:
青木生物技术(武汉)有限公司
- 宿主:
山羊
- 应用范围:
WB 1:5000-1:10000
- 规格:
100 μg/vial
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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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