- ￥803
- Sigma-Aldrich
- 进口
- D9779-1G
- 2026年05月21日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
DL-Dithiothreitol
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
3483-12-3
- 规格：
1G
属性
grade
Molecular Biology
Quality Level
200
assay
≥98% (HPLC), ≥99% (titration)
form
powder
reaction suitability
reagent type: reductant
mp
41-44 °C (lit.)
solubility
H2O: 50 mg/mL
cation traces
heavy metals (as Pb): ≤5 ppm
suitability
suitable for molecular biology
application(s)
advanced drug delivery
foreign activity
DNase, RNase, protease, none detected
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
General description
Application
这一物质可用于：
- 在蛋白质印迹分析中用作蛋白提取的组分
- 配制用于定量质谱分析的样品裂解缓冲液
- 用作酶联免疫吸附测定 (ELISA) 的激酶缓冲液成分
Biochem/physiol Actions
Features and Benefits
- 适用于分子生物学
- 不含RNA酶、DNA酶、核酸外切酶、蛋白酶
- 高纯度(HPLC ≥98%)
- 不含重金属 ≤5ppm
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Measuring protein levels in planarians using western blotting.
In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody availability and issues related to nonspecific expression. The use of western blotting can circumvent nonspecific expression, providing a dependable way to quantify proteins of interest. Here, we present a standardized, easily reproducible protocol with details on protein extractions of whole planarians and western blotting. For complete details on the use and execution of this protocol, please refer to Ziman et al. (2020a).
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
9 small, round clusters of cells. (g ) Day 12 ESC/OP9-DL1 hematopoietic cells. (h ) Day 16 ESC/OP9-DL1 hematopoietic and early T-lineage cells. (i ) Day 20 ESC/OP9-DL1 T-lineage cells. 7. Wash the plate with 4 mL of PBS
：Formerly listed as Type III 溶解性：溶于水，参考浓度2mg/ml 储存条件：2-8℃ From Sigma D1626 Salmon DNA 的配制： 组份浓度10 mg/ml Salmon DNA ;配制量约100 ml 配制方法: 1. 称取鲑鱼精DNA 2 g置于500 ml烧杯中，加入约200 ml的TE Buffer。 2. 用磁力搅拌器室温搅拌2～4小时
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
4.7H2O 1.4 mM 0.76g Mallinckrodt # 7914 dextrose (D-glucose) 15 mM 5.4 g Baker # 1916-01; Mallinckrodt Hepes (free acid) 42 mM 20 g Calbiochem # 910150 add components to 1.8 l. water pH with 10N NaOH, to pH 7.05 bring to 2l. recheck pH
技术资料暂无技术资料 索取技术资料
