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UCF.ME 鼠源RNase抑制剂(Murine RNase

inhibitor) GMP级别
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  • ¥1398
  • 翌圣生物(Yeasen)已认证
  • 10621ES10
  • 上海
  • 2025年11月11日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 英文名

      UCF.ME Murine RNase inhibitor GMP-grade

    • 保质期

      有效期1年

    • 供应商

      翌圣生物科技(上海)股份有限公司

    • 保存条件

      -20℃保存

    • 规格

      10KU

    Product Information

     

    Product Name

    Catalog No.

    Size

    Price (¥)

    Murine RNase inhibitor GMP-grade (40 U/μL)

    10621ES10

    10 KU

    1465

    10621ES20

    20 KU

    2765

    10621ES60

    100 KU

    12465

    10621ES99

    1 MU

    Inquiry

     

    Product Description

     

    RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase.

    This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can  inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.).

    This product is produced in accordance with GMP process requirements and is provided in liquid form.

     

    Product Properties

     产品细节图片1

    Source

    Recombinant E.coli with Murine RNase inhibitor gene

    Storage Buffer

    20 mM Hepes.KOH, 150 mM KCl, 8 mM DTT, 0.5%(v/v)NP 40, 0.5%(v/v)Tween20, 50%(v/v)Glycerol, pH 7.5 ±0.2(25℃)

    Unit Definition

    The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit

     

    Contents

     

    Catalog No.

    Name

    Catalog No./Specification

    10621ES10

    (10 KU)

    10621ES20

    (20 KU)

    10621ES60

    (100 KU)

    10621ES99

    (1 MU)

    10621

    Murine RNase inhibitor GMP-grade (40 U/μL)

    250 μL

    500 μL

    2.5 mL

    25 mL

     

    Shipping and Storage

     

    The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

     

    Note

     

    1. This product inhibits RNase activity in a wide range of pH, and exhibits maximum activity at pH 7-8.

    2. Avoid foaming or vigorous stirring, vortexing and other operations to prevent the product from deactivation;

    3. The product does not inhibit the activity of RNase H;

    4. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.

     

    HB220514

     

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    图标文献和实验
    该产品被引用文献
    [1] Shen W, Wang R, Fan Q, Li Y, Cheng Y. Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038/s41434-020-0151-y(IF:4.128)
    相关实验
    • 新型RNase抑制剂

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    • RNase III制备siRNA库来诱发RNAi

      有实验结果证实:用RNase III 在特定条件下降解得到的21bp 大小产物是非常有效的基因表达抑制剂。然而RNase III 完全消化产物是12―15bp 大小的片断,为了要研究这些12-15bp 大小的片断抑制基因表达的效果,一个200bp 的GAPDH 双链RNA 用RNase III 完全消化得到12-15bp 的产物并用聚丙烯酰胺胶纯化,按100nM 浓度转染Hela 细胞,另外化学合成的已知能有效抑制GAPDH 表达的siRNA 序列在同样浓度下转染Hela 细胞作为对照,相应的免疫

    • Determination of NOS Activity Using Cyclic-GMP Formation

      in the capacity of a bioassay. Whereas the half-life of NO is very short and the gas is difficult to measure during short periods of stimulation, in the presence of a phosphodiesterase inhibitor, the levels of cGMP produced in response to GC-S activation

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    文献支持
    UCF.ME 鼠源RNase抑制剂(Murine RNase inhibitor) GMP级别
    ¥1398