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大量
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北京盛科博源生物
Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure completely eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.
Brief procedure
1. Lyse tails in DirectPCR® Lysis Reagent.
2. Incubate for 45 min at 85°C.
3. PCR genotyping with 1 ml lysates.
Detailed protocols: Tail, Ear, Yolk Sac, and Cultured cells.
DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Virtually no hands-on time. Crude tail lysates for PCR!
· Money saving:
· Safe: No organic reagents.
· Environmental: No wastes (organic reagents, tubes, tips, etc...)
· Reliable and efficient: Virtually 100% success rate with high yields.
年底,现优惠促销鼠尾裂解液,现货供应
简单的操作程序,100%的成功率,保护实验室安全
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文献和实验,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
Extraction of DNA using DNAzol Reagent
without additional purification for applications such as Southern analysis, dot blot hybridization, molecular cloning, and polymerase chain reaction (PCR). 主要试剂 DNAzol® Reagent 100% ethanol 75% ethanol 8 N NaOH 实验步骤 1 Lysis
Extraction of DNA using DNAzol® Reagent
实验试剂 1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH 实验步骤 Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50
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