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上海益启生物科技有限公司
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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Basic Protein Sequence Analysis
for Understanding Results Predicting the Subcellular Localization of a Protein Using TargetP Basic Protocol 3: Predicting Key Functional Residues and Motifs using the Prosite Web Server Support Protocol 6: Guidelines
Basic Protein Chemistry Techniques
Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2 O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use, add 50 ml acetic acid to 10% final concentration. 4) Stain for 2 hours. Staining time
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