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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海益启生物科技有限公司
- 规格:
1KT
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文献和实验within 30 minutes. Membrane binding capacity: 40µg/120µg. Recovery Rate: 90-95%. Throughput: 1-24 minipreps. Low Copy Genomic DNA Isolation Kits Description: Biomoles SuperSpin Low Copy Genome DNA Isolation Kits
Genomic DNA Miniprep grow 5 ml cells over night at 30 °C spin, wash once with 1 ml H2 O resuspend in 500 µl lysis buffer add acid washed glass beads to 1.25 ml vortex 2 min
Protocol for Isolation of Genomic DNA from Mammalian Cells
Protocol for Isolation of Genomic DNA from Mammalian Cells 1.Trypsinize, harvest and resuspend cells at 107 / ml in 10 mM Tris-HCl (pH 8.0), 10 mM EDTA. 2.Add SDS and Proteinase K to a final concentration of 0.5% and 200 µg/ml, respectively
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