LN521™ - 人类重组层粘连蛋白LN521
研选

LN521™ - 人类重组层粘连蛋白LN521

收藏
  • 询价
  • BioLamina已认证
  • LN-521
  • 2025年12月13日
    售后服务
    技术支持
    文献支持
    avatar
    上海逍鹏生物科技有限公司
    代理商
    13 年钻石会员

  • 企业认证

    • 研选banner

    为原代细胞和细胞系提供细胞特异性和生物相关性的培养环境。

    研选同类产品更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 用户评价
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20度冻存

    • 保质期：

      2年

    • 供应商：

      逍鹏

    BioLamina开发、生产和销售细胞培养相关试剂，为原代细胞和细胞系提供细胞特异性和生物相关性的培养环境。Laminin-521 (LN521™)是天然干细胞环境中的关键蛋白。LN521在发育中的胚胎中表达， 在培养的人类多能干细胞（hPSCs）也会分泌。人类重组层粘连蛋白LN521化学成分限定，无异源动物成分，为干细胞再创生物相关生长环境，可长期支持细胞稳定扩增，让hPSC培养操作更可靠、更标准。完全无需添加ROCKi，即可进行简单可控的单细胞传代。细胞以均匀单细胞层生长，无需手动去除任何分化区域。

    LN521™ ----用于人类干细胞（hPSCs）培养和扩增的基质

    • 成分限定、无异源动物成分培养系统，无批次差异

    • 为细胞再创生物相关生长环境

    • 无自发分化的可靠单层细胞培养，减少工作量

    • 可单细胞传代，无需添加Rho相关蛋白激活酶抑制剂（ROCKi）

    • 无需周末加班换液，减少工作量，降低成本

    订购信息

    产品名称 产品应用 货号 规格
    Laminin-521 人类 ES 和 iPS 细胞 LN521-02 100 μg
    LN521-03

    1 mg
    LN521-05 500μg

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标用户评价
      图标暂无用户评价
      图标文献和实验
      该产品被引用文献
      人类胚胎干细胞/诱导性多能干细胞/基因组编辑(hESC/iPSC/genome editing )
      • Dynamic blebbing: A botileneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling. Weng et al., Stem Cell Research, 2018
      • Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix. Uhlin et al., J. Vis. Exp., 2017
      • Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Rodin et al., Nature Communications, 2014
      • Monolayer culturing and cloning of human pluripotent stem cells on laminin-521 based matrices under xeno-free and chemically defined conditions. Rodin et al., Nature Protocols, 2014
      间充质干细胞(MSC)
      • Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-induced Cell Sheet Technology. Jiang et al., BioMed Research International, 2016
      • CD49f Acts as an Inflammation Sensor to Regulate Differentiation, Adhesion and Migration of Human Mesenchymal Stem Cells. Yang et al., Stem Cells, 2015
      造血干细胞及血细胞(HSC and blood cells)
      • Xeno-Free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin-521. Skorik et al., Curr Protoc Stem Cell Biol. 2020
      • Superior Red Blood Cell Generation from Human Pluripotent Stem Cells Through a Novel Microcarrier-Based Embryoid Body Platiorm. Sivalingam et al., Tissue eng., 2016
      • Characterization of bone marrow laminins and identification of a5-containing laminins as adhesive proteins for multipotent hematopoietic FDCP-Mix cells. Gu et al., Blood, 1999
      内皮细胞(Endothelial cells)
      • Differentiation of Human Embryonic Stem Cells to Endothelial Progenitor Cells on Laminins in Defined and Xeno-free Systems. Nguyen et al., Stem Cell Reports, 2016
      • Integrin α6β1 Is the Main Receptor for Vascular Laminins and Plays a Role in Platelet Adhesion, Activation, and Arterial Thrombosis. Schaff et al., Circulation, 2013
      上皮细胞(Epithelial cells )
      • Chemically defined and xenogeneic-free culture method for human epidermal kera􀆟nocytes on laminin-based matrices. Tjin et al., Nature Protocols, 2020
      • Biologically relevant laminin as chemically defined and fully human pla􀆞orm for human epidermal kera􀆟nocyte culture. Tjin et al., Nat Communications, 2018
      • Abnormal Wnt and PI3Kinase Signaling in the Malformed Intes􀆟ne of lama5 Deficient Mice. Ri􀆟é et al., PLOS ONE, 2012
      • Polymerized Laminin-332 Matrix Supports Rapid and Tight Adhesion of Kera􀆟nocytes, Suppressing Cell Migra􀆟on. Kariya et al., PLOS ONE, 2012
      胰岛细胞(Pancreatic cells)
      • Culturing func􀆟onal pancrea􀆟c islets on α5-laminins and cura􀆟ve transplanta􀆟on to diabe􀆟c mice. Sigmundsson et al., Matrix Biology, 2018
      • Laminin 411 acts as a potent inducer of umbilical cord mesenchymal stem cell differen􀆟a􀆟on into insulin-producing cells. Qu et al., Journal of Translational Medicine, 2014
      • The Vascular Basement Membrane: A Niche for Insulin Gene Expression and B Cell Prolifera􀆟on. Nikolova et al., Developmental Cell, 2006
      心肌细胞(Cardiac cells)
      • In Vivo Genera􀆟on of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors. Yap et al., Cell Reports, 2019
      • Chemically defined genera􀆟on of human cardiomyocytes. Burridge et al., Nature methods, 2014
      • A Small Molecule that Promotes Cardiac Differen􀆟a􀆟on of Human Pluripotent Stem Cells under Defined, Cytokine- and Xeno-free Condi􀆟ons. Minami et al., Cell Reports, 2012
      肝脏细胞(Hepatic cells)
      • Laminin-521 promotes quiescence in isolated stellate cells from rat liver. Rohn et al., Biomaterials, 2018
      • Imaging-Based Screen Iden􀆟fies Laminin 411 as a Physiologically Relevant Niche Factor with Importance for i-Hep Applica􀆟ons. Ong et al., Stem Cell Reports, 2018
      • Recombinant Laminins Drive the Differen􀆟a􀆟on and Self-Organiza􀆟on of hESC-Derived Hepatocytes. Cameron et al., Stem Cell Reports, 2015
      • Long-Term Self-Renewal of Human ES/iPS-Derived Hepatoblast-like Cells on Human Laminin 111-Coated Dishes. Takayama et al., Stem Cell Reports, 2013
      肾脏细胞(Kidney cells )
      • The formation of quiescent glomerular endothelial cell monolayer in vitro is strongly dependent on the choice of extracellular matrix coating. Pajęcka et al., Experimental Cell Research, 2017
      • Glomerular endothelial cells and podocytes jointly synthesize laminin-1 and -11 chains. St John et al., Kidney International, 2001
      肠道细胞(Intestinal cells)
      • Designer matrices for intes􀆟nal stem cell and organoid culture. Gjorevski et al., Nature letters, 2016
      • Laminin α5 influences the architecture of the mouse small intes􀆟nal mucosa. Mahoney et al., J Cell Sci. 2008
      肌肉细胞(Skeletal muscle cells )
      • An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery. Pęziński et al., Scientific Reports, 2020
      • Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion . Penton et al., Skeletal Muscle, 2016
      • Laminin α5 chain is required for intestinal smooth muscle development. Bolcato-Bellemin et al., Developmental Biology, 2003
      神经细胞(Neural cells)
      • Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Hyvärinen et al.,Scientific Reports, 2019
      • Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model. Delsing et al., Fluids and Barriers of the CNS, 2019
      • Predictive Markers Guide Differentiation to Improve Grati Outcome in Clinical Translation of hESC-Based Therapy for Parkinson’s Disease. Kirkeby et al., Cell Stem Cell, 2016
      • The adhesion GPCR GPR126 has distinct, domain-dependent functions in Schwann cell development mediated by interaction with Laminin-211. Petersen et al., Neuron, 2015
      眼细胞(Eye cells)
      • Generation of Retinal Pigment Epithelial Cells Derived from Human Embryonic Stem Cells Lacking Human Leukocyte Antigen Class I and II . Petrus-Reurer et al., Stem Cell Reports, 2020
      • Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells. Hongisto et al., J. Vis. Exp., 2018
      • Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model. Plaza et al., Stem Cell Reports, 2015
      • Laminin-511 and -521 Enable Efficient In Vitro Expansion of Human Corneal Endothelial Cells (HCEC). Okumura et al., IVOS Cornea, 2015
      癌症细胞(Cancer cells)
      • A laminin 511 matrix is regulated by TAZ and func􀆟ons as the ligand for the a6Bb1 integrin to sustain breast cancer stem cells. Chang et al., Research communication, 2015
      • Collaboration of 3D Context and Extracellular Matrix in the Development of Glioma Stemness in a 3D Model. Ma et al., Biomaterials, 2015
      动物干细胞(Animal stem cells)
      • The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification. Boroviak et al., Nature Cell Biology, 2014
      • Laminin-511 but not -332, -111, or -411 enables mouse embryonic stem cell self-renewal in vitro. Domogatskaya et al., Stem Cells, 2008
      层粘连蛋白综述(Laminin Review)
      • Laminins in Cellular Differentiation. Yap et al., Trends in Cell Biology, 2019
      • Developing defined substrates for stem cell culture and differentiation. Hagbard et al., Phil. Trans. R. Soc. B, 2018
      • Functional Diversity of Laminins. Domogatskaya et al., Annu Rev Cell Dev Biol., 2012
       
      图标技术资料

      暂无技术资料 索取技术资料

      研选
      LN521™ - 人类重组层粘连蛋白LN521
      询价