Fluorescein isothiocyanate–dex

Labeling of Oligonucleotides with Fluorescein

Oligonucleotides are a convenient alternative to conventional, cloned DNA probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference

Fluorescein labeling of proteins

This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable

LABELING GC-GLOBULIN WITH FLUORESCEIN

Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer