isothiocyanate–dextran荧光素

产品名称
Preparation Note
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. 5kDa FITC-dextran may be used to calibrate microscale diffusion measurements. 5kDa FITC-dextran may be used to measure gut permeability in gut barrier dysfunction research.
General description
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
性质
Related Categories Alphabetical, Carbohydrates A to Z, Carbohydrates D-F, Dextrans, Fluorescent Dye-Dextran Conjugates,
更多...
mol wt average mol wt 3,000-5,000
extent of labeling 0.002-0.020 mol FITC per mol glucose
solubility H2O: soluble25 mg/mL
storage temp. 2-8°C
代理sigma全线产品，部分备有现货，欢迎来电咨询！您可以通过以下三种方式查询：
电话咨询：拨打我司全国服务热线，021-61559703，我们将竭诚为您服务！
留言咨询：请把您需要的产品货号，在网站的留言处留言，我们将会立刻给您回复！
邮件咨询：请把您需要的产品名称和货号，发送至shanranbio@163.com，我们将立刻给您回复！
好消息！公司不定期会有买产品送奖品活动，具体请咨询我司！（本活动最终解释权归本公司所有） sigma-aldrich是全球最大的化工试剂生产商,Sigma-Aldrich是一个生物/化学试剂领先的高科技企业。我们的生化试剂,有机化学试剂和试剂盒广泛应用于基因组学研究,生物科技,药物研发,疾病诊断和化工生产上.Supelco和Fluka是美国Sigma-Aldrich公司旗下的两大著名品牌,致力于分析化学领域的产品研制开发,生产销售和技术服务等。