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上海益启生物科技有限公司
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文献和实验鼠尾制备胶原蛋白-Making collagen solution from lyophilized rat tail collagen
Making collagen solution from lyophilized rat tail collagen鼠尾制备胶原蛋白 Chen-Ming Fan Lab,Carnegie Institute of Washington (lyophilized rat tail collagen from Roche) DME power from Life technologies (buy the one without NaHCO
primary fibroblasts (˜ cell number 1 x 106 ) use: A. 25 ml rat tail collagen (approx conc 2mg/ml) B. 3 ml 10xMEM C. 0.22 M NaOH: first, add 2ml, then drop-wise whilst stirring, until the collagen turns
Collagen gel containing 3T3 fibroblasts (dermal equivalent for raft culture)
75?) 1.5mL 10x reconstitution buffer 1.5mL 10x DMEM 12mL rat tail type 1 collagen (>3.8mg/mL) 10N NaOH Glacial acetic acid (in case) Method Pre-chill pipettes
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