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- 详细信息
- 文献和实验
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- 供应商:
上海益启生物科技有限公司
- 规格:
1EA
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文献和实验processes可以长达500~1000um。若去除NGF的作用,24h内processes degenerate,72h内resume multiply。passage 达70代其生物学性质也无明显改变!(来自Greene LA 1976,73:2424-2428) 2.不知flask用生长基质包被了没有,若无,PC12细胞既可呈悬浮生长又可呈贴壁生长! 3.国产的胎牛血清从质量上至多达到国外小牛血清的水平,本人建议楼主换用进口的FBS。本人应用PC12作转染试验,培养液为DMEM(high glucose
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
Fluorometric Assay for Detection of Sterol Oxidation in Liposomal Membranes
- or enzyme-induced sterol oxidation can be measured as a function of temperature and membrane composition. For the studies, two-component (e.g., phosphatidylcholine (PC)/DHE) and multicomponent (e.g., DHE/PC/bovine-brain sphingomyelin) large unilamellar
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