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文献和实验Chromosome Conformation Capture (3C) (PROT05)
which forms DNA-protein and protein-protein cross-links between regions of the genome in proximity (see figure 1 ). Subsequent restriction enzyme digestion and intra-molecular ligation produces novel junctions between restriction fragments in proximity
Magnetic Fluids Their Applications to Medicine
and quantitatively by sedimentometry. Magnetic Separation this is used in protein purification, immunoassays, pre-processing in polymerase chain reactions. There are a number of commercial systems on the market, the principal
His-tag protein purification using Ni-NTA magnetic beads
His-tag protein purification using Ni-NTA magnetic beads Materials and reagents Extraction buffer 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05
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