KAPA2G Robust HotStart PCR Kit

Bisulfite modification kits and PCR-DNA Methylation, His

I was wondering if anyone could provide an opinion on the difference in PCR amplification effiency from DNA that has been Bisulphite modified using either the MethylEasy Kit or the EZ DNA methylation kit. We are trying to amplify a PCR product

张峰手稿：基因工程技术之CRISPR-Cas9

oligo (IntegratedDNA Technology, Coralville, IA, USA) is recommended. 3. PCR reagents: Herculase II fusion polymerase (Agilent, SantaClara, CA, USA). Other high-fi delity polymerases, such asKapa HiFi (Kapa Biosystems, Wilmington, MA, USA) can alsobe

没有IRT的DNA样品或许会很脏

列的质量评估工作，证明了提取环境样品DNA的过程中使用IRT技术的重要性。一篇总结性文章曾发表于ASM 2010年年会（可致电：0755-8348 9872索取）。使用含IRT技术的MO BIO PowerSoil® DNA Isolation Kit，与不含IRT技术的竞争品牌试剂盒，提取0.1g堆肥做对比试验。最终DNA用分光光度法和凝胶电泳分析作初步分析，然后用通用引物16S rRNA，KAPA2G Fast HotStartReadymix酶跑end-point PCR。下图（图3A）展示