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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海益启生物科技有限公司
- 规格:
40 μL
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文献和实验Labeling of Oligonucleotides with Fluorescein
. They are amenable to several labeling techniques, both radioactive and nonradioactive; such methods include chemical modification during synthesis and the enzymatic incorporation of labeled nucleotides at the 5′ end or the 3′ end.
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
LABELING GC-GLOBULIN WITH FLUORESCEIN
into buffer 1. 4.Mix 50μl CFSE with the Gc-globulin solution while stirring.Let react at room temperature for 150 min in the dark (without stirring). 5.Stop reaction by addition lysine to 10 mM. 6.Desalt in a G25 column,collect fluorescent fractions
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