- ￥1012
- Sigma-Aldrich
- 进口
- 04260-5G-F
- 2026年06月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
N-Ethylmaleimide
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
128-53-0
- 规格：
5G
属性
grade
purum p.a.
Quality Segment
200
assay
≥99.0% (HPLC)
ign. residue
≤0.05%
bp
210 °C (lit.)
mp
43-46 °C
anion traces
chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤100 mg/kg
cation traces
Ca: ≤50 mg/kg, Cd: ≤50 mg/kg, Co: ≤50 mg/kg, Cu: ≤50 mg/kg, Fe: ≤50 mg/kg, K: ≤100 mg/kg, Na: ≤100 mg/kg, Ni: ≤50 mg/kg, Pb: ≤50 mg/kg, Zn: ≤50 mg/kg
storage temp.
2-8°C
SMILES string
CCN1C(=O)C=CC1=O
InChI
1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
InChI key
HDFGOPSGAURCEO-UHFFFAOYSA-N
Gene Information
human ... SLC6A3(6531), SLC6A4(6532)
Application
Biochem/physiol Actions
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文献和实验Nociceptive behavior induced by the endogenous opioid peptides dynorphins in uninjured mice: evidence with intrathecal N-ethylmaleimide inhibiting dynorphin degradation.
Dynorphins, the endogenous opioid peptides derived from prodynorphin may participate not only in the inhibition, but also in facilitation of spinal nociceptive transmission. However, the mechanism of pronociceptive dynorphin actions, and the comparative potential of prodynorphin processing products to induce these actions were not fully elucidated. In our studies, we examined pronociceptive effects of prodynorphin fragments dynorphins A and B and big dynorphin consisting of dynorphins A and B, and focused on the mechanisms underlying these effects. Our principal finding was that big dynorphin was the most potent pronociceptive dynorphin; when administered intrathecally into mice at extremely low doses (1-10fmol), big dynorphin produced nociceptive behavior through the activation of the NMDA receptor ion-channel complex by acting on the polyamine recognition site. We next examined whether the endogenous dynorphins participate in the spinal nociceptive transmission using N-ethylmaleimide (NEM) that blocks dynorphin degradation by inhibiting cysteine proteases. Similar to big dynorphin and dynorphin A, NEM produced nociceptive behavior mediated through inhibition of the degradation of endogenous dynorphins, presumably big dynorphin that in turn activates the NMDA receptor ion-channel complex by acting on the polyamine recognition site. Our findings support the notion that endogenous dynorphins are critical neurochemical mediators of spinal nociceptive transmission in uninjured animals. This chapter will review above-described phenomena and their mechanism.
Phycoerythrin conjugation protocol
DMSO Dimethyl sulphoxide, anhydrous SPDP 3-(2-pyridyldithio)propionic acid N-hydroxysuccinimide ester (Sigma) SMCC Succinimidyl trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate
Preparation of Segmented and Polarity Marked Microtubules
are microtubules with a bright seed and a dim elongated segment only on one end -- the plus end. Selective elongation of one end is achieved by inclusion of NEM-treated tubulin, a competitive inhibitor of minus end polymerization. More complex microtubule
Preparation of Segmented and Polarity Marked Microtubules
" by Sigma Bright GMPCPP Seed Mix (2 mg/ml; 1 part rhodamine tubulin to 2 parts unlabeled tubulin ; prepared and stored at -80¡C as described above) NEM GTP-Tubulin (prepared by treating recycled tubulin (~5-15 mg/ml) in BRB80 + 0.5 mM
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