Phospho-Histone H2A.X (Ser139)

Purification of mAb (IgG)

Ab concentrations.OD (Absorbance at 280 nm)/1.35 = X mg/ml.Store Abs at -20℃ or further concentrate by using Centriprep. And store at -20℃.Regenerate Ab column with Regenerate buffer (~200 ml).

Purification of mAb (IgG)

  Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

Histone H2AX Phosphorylation: A Marker for DNA Damage

) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA