- ￥6900
- GeneTex
- 美国
- GTX02872
- 2025年07月14日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX02872
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy none.
- 抗体英文名：
Hexanoyl-Lysine adduct antibody [5D9]
- 抗体名：
Hexanoyl-Lysine adduct 抗体 [5D9]
- 规格：
100 μg
WB analysis of HeLa cell lysate using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : HeLa cell lysate
Lane 3 : H₂O₂ treated HeLa cell lysate
Loading : 12 μg
Dilution : 1:1000
WB analysis of various samples using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : Hexanoyl Lysine-BSA
Lane 3 : BSA
Loading : 0.5 μg
Dilution : 1:1000
IHC-P analysis of rat heart tissue using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Dilution : 1:25
Green : Primary antibody
Blue : DAPI
Red : Actin
FACS analysis of 90% Methanol-fixed SH-SY5Y cells using GTX02872 Hexanoyl-Lysine adduct antibody [5D9]. Cells were subject to oxidative stress by treating with 250 μM H₂O₂ for 24 hours.
Dilution : 1:50
ICC/IF analysis of 5% Formaldehyde-fixed HEK293 cell using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Panel A,C,E,G : Untreated cells
Panel B,D,F,H : Cells cultured overnight with 50 μM H₂O₂
Green : Primary antibody
Red : F-actin
Blue : DAPI
Dilution : 1:50
IHC-P analysis of rat kidney tissue using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Dilution : 1:100
Green : Primary antibody
Blue : DAPI
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文献和实验An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA
Protocols for Lysine Conjugation
Currently, the most widely used chemical methodology for the conjugation of drugs to monoclonal antibodies involves either lysine or cysteine residues. In this chapter, several methods for the preparation of antibody–drug conjugates (ADCs
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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