- ￥7000
- GeneTex
- 美国
- GTX02761
- 2025年07月15日
- IHC-P
- Rabbit
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Biotinylated sheep immunoglobulin
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX02761
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
IHC-P
- 浓度：
200 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds.
- 抗体英文名：
Biotin antibody [BTN/2032R]
- 抗体名：
Biotin 抗体 [BTN/2032R]
- 规格：
100 μg
IHC-P analysis of human tonsil tissue section using GTX02761 Biotin antibody [BTN/2032R].
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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