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- TD-AB0052
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 抗体英文名：
signal transducer and activator of transcription 3
- 抗体名：
APRF; HIES; ADMIO; ADMIO1
- 规格：
25 μl/100 μl/200 μl
STAT3 mouse monoclonal antibody
| Product Name | STAT3 mouse monoclonal antibody |
| Cat.# | TD-AB0052 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.0-7.4 |
| Product Imformation | |
| Description | Anti- STAT3 mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of human STAT3 |
| Reactivity | Human ,Mouse |
| Host species | Mouse |
| Ig class | IgG2a |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | STAT3 |
| Full name | signal transducer and activator of transcription 3 |
| Synonyms | APRF; HIES; ADMIO; ADMIO1 |
| Target Background | |
| The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. | |
| Western blotting | |
| Predicted band size | 88 kDa |
| Positive control | Hela cell, Mouse brain tissue, NIH/3T3 cell, Raji cell, RAW264.7 cell lysates |
| Recommended dilution | 1000-5000 |
| Gel | 8%SDS-PAGE |
| Lysate | 40 μg |
| Lane 1-5 | Hela cell, Mouse brain tissue, NIH/3T3 cell, Raji cell, RAW264.7 cell lysates |
| Primary antibody | D191080(STAT3 Antibody) at dilution 1/1200 |
| Secondary antibody | Goat anti mouse IgG at 1/5000 dilution |
| Exposure time | 1 minute |
| Product Image | |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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