- 询价
- Tanda
- TD-AB0014
- 中国
- 2025年12月17日
- WB
- Mouse
- 脊椎动物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HA tag mouse monoclonal antibody
- 抗体英文名：
HA tag mouse monoclonal antibody
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
脊椎动物
- 保质期：
1年
- 抗原来源：
Mouse
- 级别：
科研级
- 库存：
800
- 供应商：
武汉天德
- 克隆性：
单克隆
- 保存条件：
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
- 形态：
液体
- 亚型：
IgG1
- 免疫原：
Synthetic peptide conjugated with KLH
- 规格：
100μl
HA tag mouse monoclonal antibody
Cat.# TD-AB0014
Package 25 μl/100 μl/200 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-HA tag mouse monoclonal antibody
Applications WB
Immunogen Synthetic peptide conjugated with KLH
Reactivity Fusion proteins containing the HA Tag
Host species Mouse
Ig class IgG1
Purity Protein A/G Affinity Purification
Target information
Symbol HA tag
Full name Human influenza hemagglutinin Tag
Synonyms HA epitope tag, Hemagglutinin Tag
Target Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Western blotting
Predicted band size 55 kDa
Positive control Fusion proteins containing the HA Tag
Recommended dilution 10000-50000
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文献和实验Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
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