- 询价
- Tanda
- TD-AB0012
- 中国
- 2025年12月17日
- WB
- Mouse
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
WB
- 宿主：
Mouse
- 保质期：
1年
- 抗原来源：
Mouse
- 级别：
科研级
- 库存：
800
- 供应商：
武汉天德
- 克隆性：
单克隆
- 保存条件：
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
- 形态：
液体
- 亚型：
IgG1
- 免疫原：
Synthetic peptide conjugated with KLH
- 规格：
100μl
Anti-Myc tag antibody
Cat.# TD-AB0012
Package 25 μl/100 μl/200 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-Myc tag mouse monoclonal antibody
Applications WB
Immunogen Synthetic peptide conjugated with KLH
Reactivity Fusion proteins containing the Myc Tag
Host species Mouse
Ig class IgG1
Purity Protein A/G Affinity Purification
Target information
Symbol Myc tag
Full name Myc proto-oncogene protein
Target Background
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
Western blotting
Predicted band size 55 kDa
Positive control Fusion proteins containing the Myc Tag
Recommended dilution 5000-10000
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
技术资料暂无技术资料 索取技术资料
