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- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
PBMC Fluorescent Titer Assay Kit
- 规格:
192 wells
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文献和实验With this method, antibiotic selection of the infected cells gives rise to a countable number of colonies after 7–10 d (1 –4 ). Other reporter genes are based on green fluorescent protein (GFP; ref. 5 ) or the Escherichia coli lacZ gene
peripheral blood mononuclear cells (PBMC). The assay assumes a direct relationship between expressed p24 antigen and the number of target PBMC infected. Therefore, the optimal time of p24 antigen measurement may vary due to different growth kinetics
LIVE/DEAD® Violet Viability/Vitality Kit
5 × 106 cells/mL for each assay. Cells may be suspended in medium or buffer. 3. Add 50 μL DMSO (Component C) to one vial aqua-fluorescent reactive dye (Component B). 4. Add 42 μL DMSO (Component C) to one vial calcein violet
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