- ￥7800
- Tanda
- USA
- TD-81801
- 2025年11月11日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Sar1 Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 库存：
8000
- 供应商：
武汉天德
- 规格：
30 Assays
Sar1 Pull-Down Activation Assay Kit
Cat. # TD-81801
Background
Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Sar1 is involved in membrane trafficking, and regulates the assembly and disassembly of COPII coats. Currently there is no direct assay to measure the activation of Sar1 GTPases.
The Sar1 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Sar1-GTP, but not Sar1-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Sar1 Activation Assay Kit uses configuration-specific anti-Sar1-GTP Mouse monoclonal antibody to measure Sar1-GTP levels in cell extracts or in vitro GTPγS loading Sar1 activation assays. Anti-Sar1-GTP mouse monoclonal antibody is first incubated with cell lysates containing Sar1-GTP. Next, the GTP-bound Sar1 is pulled down by protein A/G agarose. Finally, the precipitated Sar1-GTP is detected through immunoblot analysis using Anti-Sar1 Rabbit Polyclonal Antibody.
The anti-Sar1-GTP monoclonal antibody can also be used to monitor the activation of Sar1 in cells and in tissues by immunohistochemistry.
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
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