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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant full length protein of NFE2L2
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26358
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IF, IHC
- 浓度:
1mg/ml
- 抗体英文名:
Anti-NFE2L2 Mouse Monoclonal Antibody
- 抗体名:
NFE2L2 Monoclonal
- 规格:
100 μL
NFE2L2 Monoclonal
| Cat. #: TD-26358 |
| Gene Symbol: NFE2L2, NRF2 |
| Description: Anti-NFE2L2 Mouse Monoclonal Antibody |
| Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress. |
| Immunogen: Recombinant full length protein of NFE2L2 |
| Applications: ELISA, WB, IF,IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:10-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes wild type NFE2L2 protein of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Immunofluorescence:
|
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文献和实验If titre is positive do one of the following 2 weeks later: Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR Boost subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Radiolabeling of Monoclonal Antibodies
Radiolabeling of monoclonal antibodies (mAbs) is often one of the principal methods for the in vitro and in vivo assessment of these reagents, whether this is determining the affinity of a new reagent, preparing for preclinical testing
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