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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209R, human origin.
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26330
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC
- 浓度:
1mg/ml
- 靶点:
Q209R
- 抗体英文名:
Anti GNAQ(Q209R) Mouse Monoclonal Antibody
- 抗体名:
Gαq(Q209R)
- 规格:
100 μL
Gαq(Q209R)
| Cat. #: TD-26330 |
| Gene Symbol: GNAQ, CMC1, G-ALPHA-q, GAQ, SWS |
| Description: Anti-GNAQ(Q209R) Mouse Monoclonal Antibody |
| Background: GNAQ is a protein that in humans encoded by the GNAQ gene. The GNAQ protein, an alpha subunit in the Gq class, couples a seven-transmembrane domain receptor to activation of phospholipase C-beta. Mutations at this locus have been associated with problems in platelet activation and aggregation. |
| Immunogen: A synthetic peptide from the internal region of GNAQ which includes the mutation of Q209R, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes Q209R mutant, but not wild type GNAQ of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant GNAQ(Q209R) and wild type proteins. Purified His-tagged GNAQ(Q209R) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-GNAQ(Q209R) monoclonal antibody (Cat. #TD-26330). Immunofluorescence:
Immunofluorescence of cells expressing GNAQ proteins with Anti-GNAQ(Q209R) antibody. HEK293T cells were transfected with pCDNA3-GFP-GNAQ(Q209R) plasmid, pCDNA3-GFP-GNAQ (WT) plasmid, pCDNA3-GFP-GNAQ (Q209L) plasmid or pCDNA3-GFP-GNAQ (Q209P) plasmid, then fixed and stained with Anti-GNAQ(Q209R) monoclonal antibody (Cat. #TD-26330). |
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文献和实验gDNA 的影响,但这种引物设计耗时耗力,并且对于没有内含子的基因,这种方法可能就行不通了。 这时,通过 DNase 对提取的 RNA 进行预处理成为简单并且唯一的方法。诺唯赞的三代逆转录酶系列产品(R312/R323)含有 gDNA 清除模块,2 min 就能有效的清除基因组 DNA 的污染。 3. 选取合适的 RT Primer 是关键 通常 RT primer 可分为三类:oligo dT,随机引物以及基因特异性引物。根据不同的实验需求需要选择适合的引物进行使用。oligo dT
Solutions Gel Stocks Diluent 5X Buffer 25% Acrylamide 209 g Urea 209 g Urea 209 g Urea up to 500 ml Q 250 ml 10X TBE 120.8 g Acrylamide up to 500 ml Q 4.1 g BIS up to 500 ml Q 2.5 M NH4 OAc 19.2 g NH4 OAc up to 100 ml Q Formamide Dye 9 ml
过程相对简单,检测灵敏度较高;Western blotting操作过程繁琐,检测灵敏度较低。3、荧光素酶报告基因检测只需要成功将含有目标DNA序列插入到报告载体中,即可进行后续实验,Western blotting则需要购买商品化的一抗,一般价高量少,且抗体的杂交条件需要摸索;若自制的一抗,抗体效价需要验证,实验周期较长。六、参考文献Pan, Y., R. Geng, N. Zhou, G. F. Zheng, H. Zhao, J. Wang, C. M. Zhao, X. B. Qiu, Y. Q
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