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- 详细信息
- 文献和实验
- 技术资料
- 形态:
lyophilized
- 保存条件:
-20°C
- 标记物:
无
- 适应物种:
E.coli
- 库存:
大量
- 供应商:
欣博盛生物科技有限公司
- 抗体英文名:
Maltose binding protein
- 抗体名:
Maltose binding protein
- 规格:
50 µg
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文献和实验Maltose-Binding Protein as a Solubility Enhancer
as a general solubilizing agent, this has not turned out to be the case. In a direct comparison with glutathione S-transferase (GST) and thioredoxin, maltose-binding protein (MBP) was decidedly superior at solubilizing a diverse collection of aggregation-prone
Purification of MBP (maLTose-binding proteins) Fused Proteins
with 10 fractions of 10 mM maltose in 20 mM Tris pH 7.4, 200 mM NaCl, 10 mM b ME, 1 mM EDTA or in some other buffer of choice, depending on subsequent purification steps. If the fusion protein is to be cleaved with factor Xa as the next step
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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