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- TriLink
- 美国
- L-7601-100
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
At or Below -40°C
- 保质期：
包装说明
- 英文名：
CleanCap EGFP mRNA
- 库存：
大量
- 供应商：
欣博盛生物科技有限公司
- 规格：
100 ug
本文是关于CleanCap® EGFP mRNA - (L-7601)产品信息的详细介绍，如您在浏览本文的过程中，对产品有任何疑问，可联系TriLink独家代理商欣博盛生物，欢迎您前来查阅和提出宝贵的意见。
EGFP mRNA表达了一个增强型绿色荧光蛋白，该蛋白最初从维多利亚水母中获得。EGFP是一种哺乳动物细胞中常用的可直接检测的报告基因，可产生明亮的绿色荧光，发射峰为509nm。
EGFP mRNA使用了TriLink特有的共转录加帽试剂CleanCap进行加帽，该加帽试剂可产生Cap 1结构，并具有高效的加帽效率。EGFP mRNA是多聚腺苷酸化的，并针对哺乳动物系统进行了优化，模仿完全加工的成熟mRNA。
产品信息：
货号：L-7601
规格：100 µg，1 mg，5 x 1 mg
纯度：通过琼脂糖凝胶迁移实验
保存温度：-40℃或以下
长度：996个核苷酸
溶液：1 mM 柠檬酸钠溶液，pH 6.4
浓度：1.0 mg/ml
碱基组成：未修饰的碱基
换算系数：40 µg/OD260
应用：报告基因
QC检测：特性与纯度
琼脂糖凝胶迁移率：通过
浓度: ± 6% 通过
CleanCap® EGFP mRNA - (L-7601)产品信息图文对照：
关于Trilink：
TriLink BioTechnologies公司1996年成立于美国加州圣地亚哥，是世界著名的核酸修饰技术领域的研究者。TriLink致力于高品质核酸修饰产品的研发和生产，可为科研、诊断、OEM和医疗机构提供包括核苷酸、常规及特殊修饰的寡核苷酸产品，可广泛应用于基因治疗，核苷类化疗，寡核苷酸治疗和诊断领域。此外，TriLink还可提供定制、合同研究服务和ISO/ QSR标准的cGMP生产设施。TriLink完善的产品及研发服务解决方案有助于推动药物发现和生物医学研究深圳欣博盛生物技术公司是TriLink独家代理商，购买、产品咨询都可联系欣博盛生物。
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CAR-T 攻克实体瘤又出新招！超声「遥控」细胞靶向治疗癌症
可以在特定的时间和部位激活 CAR-T 细胞，从而在体内抑制肿瘤生长。该疗法的安全性高于现行的 CAR-T 疗法。图片来源：Nature Biomedical Engineering研究内容热诱导 T 细胞中的 CD19CAR 表达研究团队首先构建了一个包含热休克蛋白（Hsp）启动子的 eGFP 报告载体，这种载体在热诱导（43℃）下持续稳定表达 eGFP。为了将短暂的热刺激转化为持续的基因激活和细胞功能以发挥治疗作用，研究团队将 Cre–lox 基因开关整合到热诱导系统中，以在加热条件下表达针对 CD
1. 应用说明 AcGFP1是从水母（Aequorea coerulescens）中分离出来的荧光蛋白，是EGFP的优良的代替品。AcGFP1的特性与EGFP相似，AcGFP1的激发波峰为475nm，发射波峰为505nm。此外，AcGFP1与EGFP在核酸水平上有94%的同源性。AcGFP1的特点是具有进行了人类密码子优化的开放阅读框。这不仅提高了AcGFP1 mRNA的转化
Live imaging with Drosophila tissue culture cells
I found it sufficient to take 7 z-stacks of 1µm step size for each channel once a minute. Under these conditions exposure time should not exceed more than 100 ms in the green (eGFP) and 50 ms in the red channel (mRFP) to reduce bleaching and photo damage (see note
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