- 询价
- Cayman
- 10009277
- 进口
- 2025年07月15日
7 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Nuclear Extraction Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
上海齐源生物科技有限公司长期提供各种生物生化试剂盒价格实惠，质量有保证，信誉第一。
齐源生物提供ELISA试剂盒代测，生化试剂盒代测，木质素代测，纤维素代测服务。
齐源生物是专业生产生化试剂盒的厂家，价格优惠，现货供应。
齐源生物专业代理美国Cayman，美国Cayman公司成立于1980年，在过去20多年的发展过程中，始终保持着为全球科研工作者提供高质、优价研究产品的宗旨。该公司目前可以提供 2,138 多种生化和免疫试剂，涉及肿瘤、氧化氮、神经学、凋亡、氧化性损伤、内分泌学等研究领域。非常有特色的检测试剂盒包括类花生酸类物质、游离的生物标志、环核苷、激素及氧化氮等。此外，还提供多种高质量试剂，如类花生酸类物质、氧化氮试剂及许多相关脂质、脂肪酸、酶和抗体等。
细胞核提取试剂盒说明如下：
Purifcation of Cellular Nuclear Extracts
The following procedure can be used for a 15 ml cell suspension grown in a T75 flask
or for adherent cells grown in a 100 mm dish to 80-90% confluency, where 107
ells yields approximately 50 u of nuclear protein Adiust the volumes as needed
according to the number of cells. Keep all solutions and extracted cytoplasmic and
nuclear extracts on ice during the entire protocol.
C1. Collect cells in pre-chilled 15 ml tubes. Collect adherent cells b
scraping instead of using proteolytic enzymes.
C2. Centrifuge suspended cells at 300 x g for five minutes at 4C.
C3. Discard the supernatant. Resuspend the cell pellet in 5 ml of ice-cold
PBS/Phosphatase Inhibitor Solution and centrifuge at 300 x g for five
minutes at 4"C. Repeat PBS/Phosphatase Inhibitor Solution wash one
C4. Discard the supernatant. Add 500 u ice-cold 1X Complete Hypotonic
Buffer. Mix gently by pipetting and transfer resuspended pellet to a
pre-chilled 1.5 ml microcentrifuge tube.
C5, Incubate cells on ice for 15 minutes to allow cells to swell
C6. Add 100 ul of 10% NP-40 Assay Reagent(ltem No. 600009) Mix gently
by pipetting.
C7. Centrifuge at 14,000 x g for 30 seconds at 4@C in a microcentrifuge
Transfer the supernatant which contains the cytosolic fraction to a new
tube and store at-80°C
C8. Resuspend the pellet in 100 ul ice-cold Complete Nuclear Extraction
Buffer(1X).Vortex vigorously for 15 seconds then gently rock the tube on
ice for 15 minutes. Vortex vigorously for 30 seconds and gently rock for
additional 15 minutes
C%. Centrifuge at 14,000 x g for 10 minutes at 4C. The supernatant co
the nuclear fraction Aliquot to clean chilled tubes, keeping a small
aside for protein quantification, flash freeze and store at-80c
freeze/thaw cycles. The extracts are ready to
C10. Quantify the protein concentration using Caymans Protein Determination
Kit(Item No. 704002) or a similar protein quantification assay
NOTE: The presence of some detergents may interfere with the protein quantificatio
assay. Use the 1X Extraction Buffer as the blank and for diluting nuclear samples 1: 10
and 1: 50, and use the 1X Hypotonic Buffer as the blank and for diluting cytoplasmic
齐源生物专业代理美国Cayman其它产品
齐源生物提供ELISA试剂盒代测，生化试剂盒代测，木质素代测，纤维素代测服务。
齐源生物是专业生产生化试剂盒的厂家，价格优惠，现货供应。
齐源生物专业代理美国Cayman，美国Cayman公司成立于1980年，在过去20多年的发展过程中，始终保持着为全球科研工作者提供高质、优价研究产品的宗旨。该公司目前可以提供 2,138 多种生化和免疫试剂，涉及肿瘤、氧化氮、神经学、凋亡、氧化性损伤、内分泌学等研究领域。非常有特色的检测试剂盒包括类花生酸类物质、游离的生物标志、环核苷、激素及氧化氮等。此外，还提供多种高质量试剂，如类花生酸类物质、氧化氮试剂及许多相关脂质、脂肪酸、酶和抗体等。
细胞核提取试剂盒说明如下：
Purifcation of Cellular Nuclear Extracts
The following procedure can be used for a 15 ml cell suspension grown in a T75 flask
or for adherent cells grown in a 100 mm dish to 80-90% confluency, where 107
ells yields approximately 50 u of nuclear protein Adiust the volumes as needed
according to the number of cells. Keep all solutions and extracted cytoplasmic and
nuclear extracts on ice during the entire protocol.
C1. Collect cells in pre-chilled 15 ml tubes. Collect adherent cells b
scraping instead of using proteolytic enzymes.
C2. Centrifuge suspended cells at 300 x g for five minutes at 4C.
C3. Discard the supernatant. Resuspend the cell pellet in 5 ml of ice-cold
PBS/Phosphatase Inhibitor Solution and centrifuge at 300 x g for five
minutes at 4"C. Repeat PBS/Phosphatase Inhibitor Solution wash one
C4. Discard the supernatant. Add 500 u ice-cold 1X Complete Hypotonic
Buffer. Mix gently by pipetting and transfer resuspended pellet to a
pre-chilled 1.5 ml microcentrifuge tube.
C5, Incubate cells on ice for 15 minutes to allow cells to swell
C6. Add 100 ul of 10% NP-40 Assay Reagent(ltem No. 600009) Mix gently
by pipetting.
C7. Centrifuge at 14,000 x g for 30 seconds at 4@C in a microcentrifuge
Transfer the supernatant which contains the cytosolic fraction to a new
tube and store at-80°C
C8. Resuspend the pellet in 100 ul ice-cold Complete Nuclear Extraction
Buffer(1X).Vortex vigorously for 15 seconds then gently rock the tube on
ice for 15 minutes. Vortex vigorously for 30 seconds and gently rock for
additional 15 minutes
C%. Centrifuge at 14,000 x g for 10 minutes at 4C. The supernatant co
the nuclear fraction Aliquot to clean chilled tubes, keeping a small
aside for protein quantification, flash freeze and store at-80c
freeze/thaw cycles. The extracts are ready to
C10. Quantify the protein concentration using Caymans Protein Determination
Kit(Item No. 704002) or a similar protein quantification assay
NOTE: The presence of some detergents may interfere with the protein quantificatio
assay. Use the 1X Extraction Buffer as the blank and for diluting nuclear samples 1: 10
and 1: 50, and use the 1X Hypotonic Buffer as the blank and for diluting cytoplasmic
齐源生物专业代理美国Cayman其它产品
| (±)12(13)-DiHOME ELIA试剂盒 | (±)12(13)-DiHOME ELIA Kit | 501720 |
| 硫氧还蛋白还原蛋白酶分析试剂盒 | Thioredoxin Reductae Aay Kit | 10007892 |
| 20蛋白酶体分析试剂盒 | 20 Proteaome Aay Kit | 10008041 |
| REBP-2 转录因子分析试剂盒 | REBP-2 Trancription Factor Aay Kit | 10007819 |
| NF-kB (human p50)转录因子分析试剂盒 | NF-kB (p65) Trancription Factor Aay Kit | 10007889 |
| PPARγ 配体筛选试剂盒 | PPARγ Ligand creening Aay Kit | 10007685 |
| PPARγ 配体筛选试剂盒 | PPARγ Ligand creening Aay Kit | 10007685920 |
| 前列腺素D合成酶（人）检测ELIA试剂盒 | Protaglandin D ynthae (lipocalin-type; human) EIA Kit | 10007684 |
| 鱼样品半定量生物标记酶联免疫吸附测定试剂盒（抗鼠板) | emi-Quantitative Biomarker ELIA Component Kit For Fih ample (anti-moue plate) | 10007680 |
| 鱼样品半定量生物标记酶联免疫吸附测定试剂盒（抗鼠板) | emi-Quantitative Biomarker ELIA Component Kit For Fih ample (anti-moue plate) | 10007680 |
| 前列腺素D合成酶（人）检测ELIA试剂盒 | Protaglandin D ynthae (lipocalin-type; human) EIA Kit | 10007684 |
| 胆固醇荧光测定试剂盒 | Choleterol Fluorometric Aay Kit | 10007640 |
| 胆固醇荧光测定试剂盒 | Choleterol Fluorometric Aay Kit | 10007640 |
| 脂联素（小鼠）EIA试剂盒 | Adiponectin (moue) EIA Kit | 10007620 |
| GFAP（人类）EIA试剂盒 | GFAP (human) EIA Kit | 10007621 |
| FABP4 (human) EIA试剂盒 | FABP4 (human) EIA Kit | 10007614 |
| 脂联素（人）检测ELIA试剂盒 | Adiponectin (human) EIA Kit (H) | 10007619 |
| 抵抗素（人类）EIA试剂盒 | Reitin (human) EIA Kit | 10007610 |
| 抵抗素（大鼠）EIA试剂盒 | Reitin (rat) EIA Kit | 10007612 |
| 瘦素受体（人类）EIA试剂盒 | Leptin Receptor (human) EIA Kit | 10007608 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
以下操作按照天根产品 DP433 血液总 RNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 新鲜血液样品（ 200 μl ）2. 无水乙醇，β-巯基乙醇3. 一次性无菌注射器（DNase I配置），移液器及配套RNase-Free无菌枪头（200 μl ，1ml） 1.5 ml，2.0ml 离心管（RNase-free）4. 通风橱 涡旋振荡器 金属浴 台式低温离心机本文版权归天根生化科技
以下操作按照天根产品 DP432 植物总 RNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 植物样品（10-20 mg），研钵，液氮2. 无水乙醇，β-巯基乙醇3. 一次性无菌注射器（DNase I配置），移液器及配套RNase-Free无菌枪头（200 μl ，1ml） 1.5 ml，2.0ml 离心管（RNase-free）4. 通风橱 涡旋振荡器 金属浴 台式低温离心机本文版权归天
RNAsimple 总 RNA 提取试剂盒操作指南（DP419） ——植物
以下操作按照天根产品 DP419 RNAsimple 总 RNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 植物叶片 研钵 液氮2. 无水乙醇 氯仿3. 移液器及配套 RNase-free 无菌枪头（200 μl ，1ml），1.5 ml，2.0ml 离心管（RNase-free）4. 涡旋振荡器，台式低温离心机实验准备-试剂盒准备：第一次使用前应在去蛋白液 RD、漂洗液 RW 中
技术资料暂无技术资料 索取技术资料
细胞核提取试剂盒
询价
