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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海富雨
- 检测范围:
/
- 检测方法:
酶联免疫吸附试验法
- 应用:
检测
- 适应物种:
人
- 标记物:
HRP
- 样本:
血清、血浆、尿液、细胞培养上清、组织标本
| 中文名称 | 人钙蛋白酶1(CAPN1)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CAPN1(Calpain 1, Large Subunit) Microsample ELISA Kit |
| 别名 | PIG30; CANPL1; MuCANP; MuCL; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain |
| 货号 | ELK3861MS |
| 反应种属 | Human |
| P07384 | P07384 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.58 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of su lphuric a cid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAPN1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Neuro science;Gastroenterology; |
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文献和实验Analysis of the effect of PECAM1 on the migration and invasion of gastrointesti- nal stromal tumor cells based on single-cell transcriptome sequencing data
期刊:MODERN ONCOLOGY,Aug.2023,VOL. 31,No. 16
作者:HU Wu1,WEN Yiqiao2,QIN Jialan1,ZHAO Ying3,ZHANG Tianbiao1
DOI:10.3969 /j.issn.1672-4992.2023.16.003
个蛋白质点,获取6 张肽质量指纹图谱。通过查询数据库鉴定了9 个蛋白质。通过对过表达脊髓损伤中蛋白质表达谱改变的研究,鉴定出9 个差异表达蛋白质,这些蛋白质包括等。这些差异蛋白质涉及基因为上调蛋白钙蛋白酶Calpain、神经丝蛋白M Neurofilament tripletα12糖蛋白α12Acid2glycoprotein、Oxygen regulatedprotein 1 在脊髓损伤组下调。钙蛋白酶抑制剂Calpain inhibitor、假象蛋白210 Hypothetical
网的一种磷酸化的钙结合蛋白] calomel|甘汞 calomel electrode|甘汞电极 calorie|卡 calpactin|依钙(结合)蛋白[全称为“依赖于钙的磷脂及肌动蛋白结合蛋白”] calpain|(需)钙蛋白酶 calpain inhibitor|(需)钙蛋白酶抑制剂 calpastatin|(需)钙蛋白酶抑制蛋白 calphobindin|钙磷脂结合
MMB.Vol.176_Steroid_Receptor_Methods.rar MMB.Vol.178_Antibody_Phage_Display.rar MMB.Vol.187_PCR_Mutation_Detection_Protocols.rar MMB.Vol.189_GTPase_Protocols.rar MMB.Vol.159_Amino_Acid_Analysis_Protocols.rar MMB.Vol.144_Calpain_Methods
技术资料





