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北京诺博莱德科技有限公司
我们北京诺博莱德科技有限公司作为伯乐Bio-rad公司的经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
伯乐Bio-Rad1702909,Gel Tubes 凝胶管,产品简介:
产品品牌:伯乐Bio-Rad
产品名称:凝胶管
英文名称:Gel Tubes
产品货号:1702909
产品规格:2件装
伯乐Bio-Rad1702909,Gel Tubes 凝胶管,产品说明:
Pkg of 2, replacement part for mini prep cell (#170-2915, 170-2908)
2件装,微型准备电池更换件(#170-2915、170-2908)
491型制备电泳槽和小型制备电泳槽
491型制备电泳槽和小型制备电泳槽通过连续洗脱电泳技术,能快速有效地分离生物大分子(核酸和蛋白质)。样品经过圆柱状凝胶基质电泳,被分离为环状带,当单独的条带从凝胶底部洗脱出来时,可进行不连续液体组分收集。491型制备电泳槽或小型制备电泳槽都能分离分子量差别仅有2%的蛋白。使用这些制备电泳槽系统,您能:
- 纳克到毫克数量级的样品蛋白;分离高至500mg总蛋白;
- 通过SDS-PAGE胶,纯化分子量只相差1 ,000Da的蛋白;
- 通过非变性PAGE胶,纯化等电点只相差0.1 pH单位的分子;
- 通过琼脂糖凝胶,纯化大分子量蛋白或DNA片段(最大18kb)。
控术规格
| 491型制备电泳槽 | 小型制备电泳槽 | |
| 样品载量(质量/体积) | 1-500 mg/0.5-15 ml | 0.5-1.0 mg/50- -500 μl |
| 凝胶管直径 | 28和37mmID,14cm长 | 7mmID,13cm长 |
| 冷却 | 釉面氧化铝-陶瓷管* | 不需要 |
| 电泳仪 | 500V,40mA, 20 W (推荐PowerPac"M HV或PowerPacM通用电泳仪) |
500V,10mA, 5 W (推荐PowerPac" HV或PowerPacM通用电泳仪) |
| 洗院液G速! | 1 m/min | 0.1 ml/min |
| 所需的辅助设备 | 组分收集器,电泳仪电源,蠕动泵 | 组分收集器,电泳仪电源 |
伯乐Bio-Rad 1702926 Model 491 Prep Cell
伯乐Bio-Rad 1702927 Model 491 Prep Cell, 220/240 V
伯乐Bio-Rad 1702928 Model 491 Prep Cell without Buffer Recirculation Pump
Replacement Parts and Accessories for the Model 491 Prep Cell
伯乐Bio-Rad 1702944 Prep Cell Casting Stand
伯乐Bio-Rad 1702929 Buffer Recirculation Pump, 100/120 V
伯乐Bio-Rad 1702930 Buffer Recirculation Pump, 220/240V
伯乐Bio-Rad 1702932 Small Gel Tube Assembly, 28 mm ID
伯乐Bio-Rad 1702933 Large Gel Tube Assembly, 37 mm ID
伯乐Bio-Rad 1702934 Cooling Finger Assembly, includes feedline connectors
伯乐Bio-Rad 1702935 Buffer Circulation Tubing Kit
伯乐Bio-Rad 1702936 O-Ring Kits, 2
伯乐Bio-Rad 1702937 Dialysis Membranes, precut, 5
伯乐Bio-Rad 1702938 Frit Kit, includes support frit and elution frit
伯乐Bio-Rad 1702939 Sample Application/Overlay Buffer Kit
伯乐Bio-Rad 1702940 Thumbscrews, 4
伯乐Bio-Rad 1702969 Lid with Power Cables
伯乐Bio-Rad 1615101 Prep Cell Starter Kit
伯乐Bio-Rad 1610323 Prep Cell Starter Kit Protein Standard, 1 ml
伯乐Bio-Rad 1702941 Elution Manifold Base
Mini Prep Cells
伯乐Bio-Rad 1702915 Mini Prep Cell with Reagent Starter Kit
伯乐Bio-Rad 1702908 Mini Prep Cell without Reagent Starter Kit
Replacement Parts and Accessories for the Mini Prep Cell
伯乐Bio-Rad 1702909 Gel Tubes, 2
伯乐Bio-Rad 1702913 Sample Application/Purge Kit
伯乐Bio-Rad 1702947 Peristaltic Pump Adaptor Kit, for 0.8 mm tubing
伯乐Bio-Rad 1702948 Elution Frit Kit. with 5 dialysis membranes, MW cutoft3,500
伯乐Bio-Rad 1702911 Elution Frit Kit
伯乐Bio-Rad 1702912 Harvest Ring Assembly
伯乐Bio-Rad 1702917 Mini Prep Cell Elution Chamber Top
伯乐Bio-Rad 1702918 Mini Prep Cell Casting Stand
伯乐Bio-Rad 1702916 Elution Manifold Base
伯乐Bio-Rad 8007533 Lid with Cables, for mini prep cell
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文献和实验SDS software (Applied Biosystems). 3% ReadyAgarose Precast Gel (Bio-Rad). Agarose gel electrophoresis apparatus (Bio-Rad). Detailed procedure Reverse Transcription
Procedure: (This protocol uses anti-XKCM1 for immunodepletion.) Put 25 ul of Bio-Rad Affi Prep bead slurry into two 0.5 ml tubes labeled IgG and XKCM1. Wash beads 3X with 0.5 ml TBST
. These charged acids can be removed by passage through a mixed-bed resin (e.g. Bio-Rad AG 501-X8). This must be done quickly, minimizing exposure of the glyoxal to air, or it will just reoxidize as you work! Our method is to use a baked spatula to load
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