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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Cleavable Biotin Alkynes, Sample Pack
- 供应商：
靶点科技
- 规格：
1 pack
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文献和实验Preparation and purification of biotin-labeled probe for ISH
; and electrophorese the sample on a mini-gel with a DNA size marker. The size of the nick translation product can be adjusted by adding a different amount of DNase I in the reaction solution. Random primer labeling also can be used for biotin-labeling
Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
the 1.5 ml tube “cap” to allow the matrix to settle above the cotton-plug stop, while permitting liquid to pass through. After the matrix has filled the pipet pack it down with a continuous flow of TEAB for 10-15 min. This can be most easily accomplished
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