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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
AFDye 350 Azide
- 供应商:
靶点科技
- 规格:
5 mg
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文献和实验Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
two hours. Add 350 m l Stop Solution. Incubate at 37°C for 30 min. (optional). Add 350 m l PCI-9, mix by vortexing, spin in microfuge 30s, transfer the supernatant to a new tube and repeat xl (2 PCI extractions total). Add 350 m l
. Incubate at 37℃ for two hours. Add 350 ml Stop Solution. Incubate at 37℃ for 30 min.(optional). Add 350 ml PCI-9,mix by vortexing,spin in microfuge 30s,transfer the supernatant to a new tube and repeat xl (2 PCI extractions
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