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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Fluorescein-Biotin-Azide
- 供应商：
靶点科技
- 规格：
1mg
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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
A general protocol for staining cell for cytometry analysis
mix the cells and incubate for 15 min at RT in the dark. *For Annexin V-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1X Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 13024D) into 100
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