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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Cy5-Biotin-Azide
- 供应商：
靶点科技
- 规格：
1 mg
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文献和实验Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
PBS/azide. Resuspend the cells in propidium iodide at 50 µg/ml in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for FITC, PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
Description This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
Dual- and Triple-Color Fluorospot
is the availability of additional amplification systems compatible with, e.g., FITC/anti-FITC and biotin/avidin and, in particular, automated readers designed for the analysis of three-colored spots. One amplification strategy that has been evaluated is to use
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